In order to follow tumor growth non-invasively in a syngeneic mouse model of PDA, we generated Panc02 cells expressing eGFP and firefly luciferase (Panc02-FUGLW) and injected 1×104 of these tumor cells orthotopically into albino wild type C57Bl/6 mice and albino C57Bl/6 mice deficient for TNF, TNFR1, TNFR2 or both TNFRs. Here, TNFRSF1A is linked to Patent ductus arteriosus.