qRT-PCR data indicated that expression of PRDX4 mRNA increased almost two fold higher in the matured follicle GCs after IVM than immature GCs, the difference was significant (p<0.05) (Figure 3A, PCOS IVM vs. PCOS SFs), and nearly the same level as in vivo matured follicle GCs of PCOS patients (p>0.05) (Figure 3A, PCOS IVM vs. PCOS LFs). This evidence concerns the gene PRDX4 and polycystic ovary syndrome.