EGF and infection: Notably, prolonged ablation of Vps34 for an additional 4-5 days (day 14-15 post-infection) consistently resulted in cell vacuolization (Figure S1C), a breakdown of the lysosomal compartment (as evidenced by an aberrant LAMP1 compartment) (Figure S1C) and impaired lysosomal degradation of the EGF receptor in response to EGF stimulation (Figure S1D).