Notably, prolonged ablation of Vps34 for an additional 4-5 days (day 14-15 post-infection) consistently resulted in cell vacuolization (Figure S1C), a breakdown of the lysosomal compartment (as evidenced by an aberrant LAMP1 compartment) (Figure S1C) and impaired lysosomal degradation of the EGF receptor in response to EGF stimulation (Figure S1D). This evidence concerns the gene PIK3C3 and infection.