The samples classified in the latter group by MDA are selected for HbA2 measurement; based on the values obtained and the presence or not of Hb variants, molecular analysis can be performed, but the results obtained in the mixed group suggest that the mixed thalassemia and iron deficiency status remain the most difficult to detect, and 19.5% of the patients with genetic anemia were misclassified as IDA. Here, GSTM1 is linked to thalassemia.