To confirm our findings in zebrafish using an established mammalian model of polyposis, we examined GSK-3 and mTORC1 activity in Apcmin mice, which develop intestinal adenomas through loss of heterozygosity (LOH) for Apc. Phospho-S6 staining was strongly induced in polyps compared with adjacent normal tissue (Fig. 6A), similar to previous reports (Fujishita et al., 2008; Metcalfe et al., 2010). The gene discussed is APC; the disease is polyposis.