Although 5-FU has been reported to induce apoptosis via the Fas pathway in liver metastases of colorectal cancer patients,56 and stimulate p-JNK in colorectal cancer cells,57 our current results suggest the probable existence of a Fas/JNK-independent mechanism in chemotherapy of 5-FU, as well as the importance of Fas/JNK pathway in protection against apoptosis by ADMA. This evidence concerns the gene MAPK8 and colorectal cancer.