TNF and colorectal adenocarcinoma: We found that TPKC also significantly diminished TNF-induced necroptosis in murine NIH3T3 fibroblasts cells as well as in human leukemic Jurkat T cells and in human HT-29 colorectal adenocarcinoma cells (Figure 1A) as further established cell systems for necroptosis[14,15,34].