In additional effort, we preformed in vitro combination of AZD5363 with the commonly used chemotherapy agents in GC including Taxotere, SN-38 and Oxaliplatin in a number of GC cell lines with both PI3KCA mutation and PTEN loss (HGC27), PI3KCA mutation alone, and PI3K and PTEN wild-type status. The gene discussed is PTEN; the disease is gastric cancer.