To partially evaluate whether the multiplex MIA demonstrated flavivirus cross-reactivity greater or less than the standard screening ELISA, an Arbovirus Diseases Branch database search was performed for IgM and IgG ELISA results of sera from confirmed SLE cases, because SLE represents one of the most serologically cross-reactive viruses in the genus. This evidence concerns the gene CD40LG and systemic lupus erythematosus.