Following transient transfection of GLI3R into HT29 (densitometric quantification in Fig. 2A) and HCT116 (Fig. 2B) colon cancer cell lines, GLI1, GLI2 and hTERT protein levels were decreased over a period of 48 hr – 72 hr. The gene discussed is GLI2; the disease is malignant colon neoplasm.