Additionally, immunohistochemical analysis of the brains of 117VV Tg30 mice inoculated with GSS A117V prion isolate I514 all showed pathological lesions characterised by gliosis (Figure 1L) and spongiosis (Figure 1J) that reflected the level of PrP plaques (Figure 1K) deposited in a similar pattern to 117VV Tg31 mice described above. This evidence concerns the gene PRNP and Gliosis.