The forward stepwise mode constructed discriminant functions including all genes under study, and showed that DMT1, HAMP, TfR2, miR-31, miR-149, miR-182, miR-194 were the most significant parameters responsible for progression of colorectal adenocarcinoma, assigning all cases correctly (p < 0.0001). The gene discussed is HAMP; the disease is colorectal adenocarcinoma.