TRPV4 and ischemia: Based on the fact that ischemia and the first hours of reperfusion are associated with a strong release of 5, 6-EET, which has been proven to be an endogenous TRPV4 agonist [13], we investigated the effects of TRPV4 blockade on intestinal inflammation induced by 50′ minutes of ischemia, followed by 5 hours of reperfusion.