Using CBA, we found that VEGF protein in RCC cell lines was detected mainly in the conditioned medium, not in cell-lysates; conversely, bFGF protein was detected mainly in cell-lysates, not in the conditioned medium, and total VEGF expression was higher in 786-O cells than in OUR-10 cells, whereas bFGF was more highly expressed in OUR10 (Figure S1). The gene discussed is VEGFA; the disease is renal cell carcinoma.