To test this, we first analyzed the expression of PINT by qRT-PCR in p53+/+ and p53-/- matched HCT116 human colorectal cancer cell lines [33], and found that PINT was induced in p53+/+, but not p53-/- cells when treated with the DNA-damaging drug 5-fluorouracil (5-FU) (Figure 6A,B). Here, TP53 is linked to colorectal cancer.