Notably, immunohistochemistry (IHC) and enzymatic staining revealed a progressive increase in the relative presence of intestinal differentiation lineages, namely Goblet (Periodic Acid Schiff; PAS), Paneth (lysozyme), and entero-endocrine (synaptophysin) cells in the transplanted intestinal tumours when compared with the primary Apc1638N/+/KRASV12G lesions (Figure 1c). This evidence concerns the gene LYZ and intestinal neoplasm.