qRT-PCR was performed for each of the 14 new genes, as well as for 5 standard control genes (GAPDH, ACTB, TBP, HPRT1, ABL1), on cDNA from a panel of 14 leukemia samples (10 AML, 4 ALL) plus one CD34+ cord blood sample (using equal amounts of RNA). Here, ACTB is linked to leukemia.