The band corresponding to YB-1 (50 kDa) of three cancer cell lysates (breast cancer cell, MDA-MB-231; HCC cell, HepG2; and NSCLC cell, A549) and recombinant YB-1 were recognized by the commercial YB-1 mAb, the prepared 1-D9, and the YB-1 pAbs, respectively (Figure 3). The gene discussed is YBX1; the disease is breast cancer.