To further assess the contribution of NKT cells and AM in the production of IL-5 by c-kit+ ILC2 in the IAV infected lungs we analyzed the impact of selective depletion of NKT cells and/or AM from 12 d.p.i. total lung cell suspensions prior to 24-hour ex vivo culture on the frequency of IL-5+ c-kit+ ILC2 (the predominant ILC2 type at this time after infection). Here, KIT is linked to infection.