To test whether FGFR1 induces phosphorylation of PI3K p85, Akt1, and BAD in vivo, we suppressed FGFR1 expression by direct injection of FGFR1 siRNA to the left ventricular anterior wall of wild-type mice as descried above with a scrambled siRNA as a control and induced myocardial ischemia/reperfusion injury at 3 days after siRNA administration. The gene discussed is FGFR1; the disease is myocardial ischemia.