The replication of encephalomyocarditis virus (EMCV, a positive ssRNA virus) was also inhibited, five-fold by high levels of wild-type STAT1/STAT2/IRF9 or four-fold by Y701F-STAT1/STAT2/IRF9, when assayed 6 h after infection (Figure 3A, right panel). This evidence concerns the gene STAT1 and infection.