To see whether the proportion of PrP226* in deposits (i.e. the D/N ratio in PrP226* assay) correlates with standard CJD sample classifications, D/N ratio measured by PrP226* assay was compared to the PrPSc type, to the Met/Val polymorphism at the codon 129, to the presence or absence of 14-3-3 protein in the cerebrospinal fluid (CSF) and to the PrPSc deposition pattern, determined by the immunohistochemical evaluation of CJD/GSS samples. The gene discussed is YWHAQ; the disease is Creutzfeldt Jacob disease.