INS and Creutzfeldt Jacob disease: To investigate whether the low D/N values of the first group of CJD/GSS samples were caused by insufficient homogenization or denaturation of the samples, we repeated the measurements of these samples after additional homogenization by passage through the insulin syringe with 31G needle and by their denaturation at higher temperature (ie. by 1.5 M Gdn-SCN for 10 min at 90°C).