Western blot analyses of sCJD and non-CJD brain homogenates were performed with two anti-PrP mAbs, 6H4, that reacts with the epitope PrP144-152 and V5B2, that reacts with the epitope PrP214-226, with last three amino acids AYY being crucial for binding (Figure 1A). The gene discussed is PRNP; the disease is Creutzfeldt Jacob disease.