POU3F2 and melanoma: In two different NZM melanoma cell lines (NZM11 and NZM12), one of which has previously been characterized to be a “lower MITF” cell line (NZM11), and the other cell line previously characterized as a “higher MITF” cell line (NZM12) (10, 26), the knockdown of PAX3 expression resulted in decreased levels of POU3F2 transcripts in both cell lines (Figure 5).