To investigate whether inhibitors of COX1 and COX2 could prevent RSV replication, up-regulation of claudin-4 and occludin, and expression of proinflammatory cytokines, HNECs were pretreated with 0.1–10 μg/ml both inhibitors 30 min before infection with RSV at an MOI of 1 for 24 h. The gene discussed is CLDN4; the disease is infection.