For the same, the effect of calcarea carbonica in different tumor cells (wild-type-p53 expressing cancer cells EAC, S-180, MCF-7, HBL-100 and p53-mutated MDA-MB-231) was determined in in vitro conditions by exposing tumor cells with 20 μl/ml of calcarea carbonica for a period of 96 h. This evidence concerns the gene TP53 and cancer.