The nuclei of the nerve fascicles in which the NK-1R immunoreactions described above were noted and which thus were located in myositis areas showed, when DAPI was used in embedding medium and when S100beta immunolabelling was performed, another colour reaction than the nuclei located outside the nerve fascicles (Figures 6(c) and 6(f)). This evidence concerns the gene S100B and myositis disease.