Specificity of the effects was further confirmed by RNA interference and IκBα (Ser32, Ser36 to Ala32, Ala36) mutant analysis in HeLa cells (Figure 3) and A549 cells (Figure 5) in addition to the wt-MEF and MCF 7 breast cancer cells (Additional file 2: Figure S2). This evidence concerns the gene NFKBIA and breast cancer.