As ID8 cells express the mesothelin (Additional file 3: Figure S2), a well-characterized tumor antigen [8], we harvested splenocytes from 2 mAb and control mAb treated mice, and the same number of splenocytes was cultured in the presence of 10 μg/mL of H-2Db-restricted mesothelin-specific peptide (amino acid 406–414) or control HPV-E7 peptide (amino acids 49–57) for 3 days and assayed the culture supernatants for IFN-γ by ELISA. The gene discussed is MSLN; the disease is neoplasm.