To determine the cytokine expression of respiratory DC subsets during Klebsiella pneumonia we sorted pDC, CD103+ DC, CD11b+ DC and CD11c+ MHC-class IIlow cells from Klebsiella-infected animals (d5 p.i.)to high purity (>98%) and analysed mRNA expression by quantitative real time RT-PCR. The gene discussed is ITGAM; the disease is Klebsiella pneumonia.