Cx43 expression was heterogeneous in different tissue samples: in well differentiated gastric cancer tissues, Cx43 expression showed a major mixed (cytoplasmic and membranous) staining (Figure 1B); moderately differentiated gastric cancer tissues had only cytoplasmic Cx43 expression (Figure 1C); and Cx43 was scarcely expressed in poorly differentiated gastric cancer tissues (Figure 1D). This evidence concerns the gene GJA1 and gastric cancer.