The studies supporting SOP development are summarized in the online Supporting Information, including examples of CF and non-CF tracings (Figure S1 in File S1), the effects of CFTR blockers on CFTR currents (Figure S2 in File S1), the impact of indomethacin on ICM parameters (Figure S3 in File S1), comparison of buffer conditions (Figure S4 in File S1), and stimulation with CFTR-activating conditions (Figure S5 in File S1). The gene discussed is CFTR; the disease is cystic fibrosis.