Overexpression of ZIP5 in N2a neuroblastoma cells followed by treatment with PNGase F and immunoblot analyses established that ZIP5 can also be N-glycosylated (Figure 1C), including at a highly conserved N-glycan acceptor site also known to be occupied in PrP and ZIP10 (not shown) [12]. This evidence concerns the gene PRNP and neuroblastoma.