TMEM74 and neoplasm: To replicate these findings we investigated whether the five candidate methylated genes (GCM2, RGS7, TMEM74, NEFM and AEBP1) were also methylated in the 199 tumour and normal pairs analysed on the same platform (Illumina HumanMethylation27 array) by the Cancer Genome Atlas (TCGA) Kidney renal clear cell carcinoma (KIRC) project.