Therefore, in this study, we investigated the roles of RIG-I and MDA5 in CSFV infection in vitro and examined activities of IFNs and inflammatory cytokines in order to elucidate the mechanism of the virus infection in host, the results demonstrated that both RIG-I and MDA5 were essential and sufficient for the activation of transcription factors IRF-3 and NF-κB which induced the normal antiviral and inflammatory responses to CSFV. Here, IRF3 is linked to viral infectious disease.