In addition, the mRNA expression levels of ANP and β-MHC, which are cardiac hypertrophy-related genes, were increased by 25.76 ± 6.65 and 4.53 ± 0.43 fold, respectively, after the incubation with Ang II in WT-H9c2 cells but not in TG-H9c2 cells (Figure 2d and e). Here, NPPA is linked to cardiac hypertrophy.