It is known that sclerostin is a secretory protein thenshould be secreted into conditioned media of MM cellline (U266) and when we add this condition mediato osteogenic medium consisting of UCSC, it inhibits osteoblast differentiation and RUNX2 as essentialmarker for osteoblast lineage is not expressed becauseRunx2 is an essential factor for expression of collagen type I, Osteopontin and bone sialoprotein (9, 22).Therefore we could not detect RUNX2 expression after 21 days culture UCSC in osteogenic medium consisting of myeloma cell line condition media (Fig 4). This evidence concerns the gene IBSP and Miyoshi myopathy.