Another study using maternal high-fat (saturated or trans fat) feeding from 4 weeks prior to mating through until weaning, showed that while a diet high in saturated fat increased circulating leptin levels in both male and female offspring, anxiety, as assessed on the elevated plus maze (EPM), was increased only in male rats and apparently irrespective of the composition of maternal high-fat feeding [18]. This evidence concerns the gene LEP and Anxiety.