MAPT and infection: As shown in Figure 5, immunoblot analysis demonstrated that Tau dissociated from microtubules following infection of endothelial cells with P. aeruginosa ExoY+, with significantly less tau bound to microtubules in ExoY intoxicated cells relative to the amount bound in control and K81M treated cells [0.22±0.09 vs. 0.44±0.08 (Ctr) and 0.46±0.04 (K81M); n = 4; P<0.05 compared to both K81M and untreated cells].