CD4 and hepatocellular carcinoma: The results showed that CD4+ Foxp3+ Tregs gradually accumulated in the tumor tissue of early-stage HCC on day 8 and 14 after Hepa1-6 sc inoculation (Figure 1A), CCR7 downregulation and Foxp3 upregulation in the development of HCC from day 8 to 29 were also verified (Figure 1B).