These data suggested that functional capacity of tumor-infiltrating effector cells was markedly enhanced after Tregs depletion in CCL21-treated mice, infiltration of activated CD4+ T cells and DCs in the Tregs-deprived tumor microenvironment might promote CD8+ T cells function, leading to effective T-cell priming and the generation of powerful antitumor immune responses. Here, CCL21 is linked to neoplasm.