To confirm the results of the colorectal cancer cell line studies, we evaluated Livin expression at the RNA and protein levels by RT-PCR, Western blotting and immunohistochemistry in human colorectal cancer tissues, paired normal colorectal mucosa, and metastatic and non-metastatic lymph node tissues of same patients taken from colonoscopic biopsy and surgical specimens. This evidence concerns the gene BIRC7 and colorectal cancer.