As previous studies have demonstrated that both TGF-β and PI3K/AKT pathways are critical for EMT in cancer cells including those from HCC [3], [5], [16], we firstly analyzed whether p53 has a functional role on TGF-β- and PI3K/AKT-induced EMT in PVTT-1 cell. This evidence concerns the gene AKT1 and hepatocellular carcinoma.