Compared to normal liver cells L02, which displayed a modest methylation level (39%), DNA hypomethylation of NANOG (Figure 1A–i) was observed in three liver cancer cell lines, namely PLC, 97L and 97H (18%, 8% and 14%, respectively), among which the metastatic liver cell line 97L showed nearly absence of DNA methylation (Figure 1B and 1C). Here, NANOG is linked to liver cancer.