For the c-MYC gene, although the CpGs located upstream of TSS1 and TSS2 and within exon 2 remained unmethylated in both normal and cancer cells (Figure 1A–iii; Figure S2 in File S1), the methylation level of exon 3 was dramatically reduced and became hypomethylated in two liver cancer cell lines, PLC (34%) and 97L (6%), when compared to the moderate to high level of methylation (51% to 82%) in normal liver cells (Figure 1F). This evidence concerns the gene MYC and liver cancer.