To determine whether TLRs 2 and 9 also control neutrophil recruitment throughout 3 weeks of repeated exposures (at which time granulomas develop) we exposed WT, TLR2-/-, TLR9-/-, and TLR2/9-/- mice to SR 3 times / week for 3 weeks and performed BAL one day after the last exposure. The gene discussed is TLR2; the disease is Granuloma.