In order to confirm that cyclin A1 is also over-expressed at the protein level in primary myotubes, we performed Western blot analysis and found it clearly up-regulated (>3-fold higher) in FSHD patients (n = 4) vs. age-matched healthy controls (n = 4) (Fig. 4A, 4B). This evidence concerns the gene CCNA1 and facioscapulohumeral muscular dystrophy.