PAX4 and obesity due to melanocortin 4 receptor deficiency: However when analyzing the overall distribution of PAX4 expression, we observed a statistically significant decrease in the percentage of PAX4+ alpha cells per islet from the T2D cohort compared to individuals with obesity (respectively 37.2±3.7% vs 52.8±4.8%) (Figure 5B), whereas there was no significant change in the percentage of beta cells expressing PAX4 per islet in the same patients (Figure 5C).