In order to compare the hormone receptor expression assessed by two IHC methods distant in time and investigate the prognostic implications of using various cut-off values to define ER and PR positivity, we decided to make the reappraisal of ER and PR status, using current IHC methodology, on the same previously analysed tumour samples of patients with breast cancer diagnosed in the 90’s in our Institution. This evidence concerns the gene NR4A1 and breast carcinoma.