GPLD1 and autosomal dominant polycystic kidney disease: Figure 5B shows that after 48 h exposure to 10 μM PLD1- and 20 μM PLD2-inhibitors autophagosomes were abundantly present. To further examine the formation of autophagic vesicles, we performed immunofluorescence analysis for LC3. As expected, LC3 aggregated substantially in response to PLD inhibitors in both ADPKD and OX161 cells (Figure 5C).