As shown in Fig. 5, based on the expression level of internal control β-actin, several human cancer cell lines such as HepG2 (hepatocellular carcinoma), SKBR3 (breast cancer), SKOV3 (ovarian carcinoma) and A549 (lung epithelial adenocarcinoma) exhibited a strong reactivity to anti-Prdx1. This evidence concerns the gene ACTB and breast cancer.