In another study with HCC, only 2 of the 70 (2.9%) HCC sera showed a positive response to Prdx1, and 1 of 70 (1.4%) normal human sera was positive to Prdx1, as detected by ELISA with phage-expressed Prdx1 protein as the coating antigen (29). Here, PRDX1 is linked to hepatocellular carcinoma.