By performing rapid amplification of cDNA ends (RACE) on total human brain RNA followed by next generation sequencing, we have identified two new transcripts that we refer to as FMR5 and FMR6. The expression of these newly described RNA species was validated in several regions of unaffected human brain tissue as well as in brain samples from FXS and premutation carriers. The gene discussed is FMR1-AS1; the disease is fragile X syndrome.