To determine the suppression of PPP2R5C expression in CML cells after siRNA treatment, PPP2R5C mRNA expression was analyzed by qRT–PCR 24, 48, and 72 h after nucleofection, while the suppression of PPP2R5C protein expression in K562 and K562R cells was analyzed by immunoblotting 72 h after nucleofection. The gene discussed is PPP2R5C; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.